Department of Biotechnology & Molecular Biology,
College of Basic Sciences & Humanities
CCS Haryana Agricultural University Hisar, Haryana
A Practical Training Course on TECHNIQUES IN TISSUE CULTURE, GENETIC ENGINEERING AND MOLECULAR BIOLOGY
May 12th, 2010 to June 23rd, 2010
Objective
College of Basic Sciences & Humanities
CCS Haryana Agricultural University Hisar, Haryana
A Practical Training Course on TECHNIQUES IN TISSUE CULTURE, GENETIC ENGINEERING AND MOLECULAR BIOLOGY
May 12th, 2010 to June 23rd, 2010
Objective
Agricultural biotechnology encompassing areas of transgenic development, structural and functional genomics including marker assisted crop improvement could provide us with opportunities towards breaking the yield plateaus for sustainable food production. The recombinant DNA technique has enabled gene transfer across all possible inter-species barriers. The present era of ‘Gene Revolution’ requires skilled human resources in this high throughput area. The Department of Biotechnology & Molecular Biology, CCS Haryana Agricultural University, Hisar, Haryana is a leading seat of learning in the field of Agricultural Biotechnology with emphasis on plants and microorganisms. The department is going to organize a six weeks hands on training on the topic topic “Techniques in Plant Tissue Culture, Genetic Engineering and Molecular Biology ” covering different techniques in plant tissue culture, genetic engineering and molecular biology. The proposed training has been designed keeping in view the needs of State Univ. & Colleges and Graduate college students. The training programme will include lectures as well as practical classes.
Course contents
-Isolation and purification of genomic DNA and RNA from plants and microorganisms.
-Estimation of quality and quantity of nucleic acids by agarose gel electrophoresis and/or UV spectrophotometer.
-PCR amplification of genomic DNA using RAPD/ISSR/SSR primers.
-Resolution of PCR amplified products by agarose gel electrophoresis.
-Scoring of amplified bands and analysis for polymorphism/sexspecificity.
-RT-PCR and Cloning of RT-PCR products in E.coli.
-Agrobacterium-mediated genetic transformation of different crop species such as rice, Brassica etc.
-Estimation of quality and quantity of nucleic acids by agarose gel electrophoresis and/or UV spectrophotometer.
-PCR amplification of genomic DNA using RAPD/ISSR/SSR primers.
-Resolution of PCR amplified products by agarose gel electrophoresis.
-Scoring of amplified bands and analysis for polymorphism/sexspecificity.
-RT-PCR and Cloning of RT-PCR products in E.coli.
-Agrobacterium-mediated genetic transformation of different crop species such as rice, Brassica etc.
Course Fees: Participants are requested to pay a sum of Rs. 10000/- (Rs Ten thousand only) as registration fee. The registration fee shall be deposited in cash at the time of registration on May. 12th, 2010 on the office of BMB Deptt.Laboratory and Computing facilities: The biotechnology & molecular biology laboratories are well equipped with modern equipments and other lab wares required. Lodging and Boarding: Arrangements for the stay of the student participants during the training program will have to be made by the students on their own.
The organizers of the course will not bear any expenses on account of boarding and lodging of the participants. Number of Participants: The maximum number of participants shall be 20.
Participants and Eligibility: Participants are invited from ICAR Institutes/ SAU/Basic Science Institutes/ Sate Governments/Private Organizations. They should be graduates or Graduation students having interest in the training.
How to apply: The application for participation may be sent in enclosed prescribed format, duly forwarded by Head of the institution. It should reach the Course Director latest by May. 11th, 2010 by post, fax or email.
TA & DA of the participants will be borne by participants/sponsoring institutions/ organisations etc.
The participants will be selected on ‘first come first serve’ basis and shall be registered on 12-05-2010.
All correspondence may please be addressed to:
Dr. S Dhillon, Course Director cum Prof & Head
Department of Biotechnology & Mol Biol, CCS HAU, Hisar
Phone no. 01662- 289407 (O) Cell: 09416102349 Fax 01662-234613, 234952
Email: hod_bmb@hau.ernet.in, santosh_dhillon@hau.ernet.in,
santosh_dhillon@rediffmail.com
Department of Biotechnology & Mol Biol, CCS HAU, Hisar
Phone no. 01662- 289407 (O) Cell: 09416102349 Fax 01662-234613, 234952
Email: hod_bmb@hau.ernet.in, santosh_dhillon@hau.ernet.in,
santosh_dhillon@rediffmail.com
Photocopy of the application form can be used
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